Jong-Young Kwak, Ph.D.
Professor, Department of Pharmacology & Immune Network Pioneer Research Center,
Ajou University School of Medicine,
Koreajykwak@ajou.ac.kr3dajou@gmail.com
Biography:
Dr. Kwak is professor at Department of Pharmacology, Ajou University School of Medicine, Korea. He is currently the Director of Immune-network Pioneer Research Center, Ajou University Medical Center. He became a vice-president of Korean Socie-ty of Biochemistry and Molecular Biology in 2015 and is a Doctor Honoris Causa in Russian Academy of Science since 2012. He completed his doctorate in Medical Bio-chemistry with neutrophil activation and signal transduction pathways at the Pusan National University, Korea in 1991. After his study of activation of neutrophils in Emory University as a post doctorate, he directed his research to dendritic cell analy-sis. Current research topic in his laboratory is immunogenic responses in 3D culture of immune cells and tissues. He is CEO of venture company, Nanofaentech Inc. in Korea.
Topic title：Three Dimensional Coculture Models of Immune Cell Responses Using Nanofibrous Membrane
Abstract：In this
study, we developed nanofibrous scaffold-based model mimicking numerous diseases associated with
disorder of immune responses such as cancer, inflammation, and autoimmune
diseases. First, when dendritic cells (DCs) were
co-cultured with mitoxantrone-treated CT26 colon cancer cells in a polycaprolactone
(PCL) nanofibrous scaffold, BM-DCs sprouted cytoplasm to, migrated to, synapsed
with, and engulfed mitoxantrone-treated CT26 cancer cells, which were similar to the
naturally occurring cross-talk between these two types of cells. Second, the migration of phagocytes to S. aureus was evaluated in a PCL nanofibrous membrane-based
two-layer culture system. Neutrophils, macrophages, and DCs cultured in the
upper layer migrated to the lower layer containing bacteria. Third, artificial
asthma model was developed using two-layer culture system with lung epithelial
cells cultured on upper poyl(vinyl) alcohol (PVA) nanofibrous membrane, DCs cultured on lower PCL nanofibrous
membrane, and Der P, a component of dust mites. DCs are one of the main sources
for IL-23 secretion. The production of IL-23 and
IL-6 but not pro-inflammatory cytokines such as IL-1b and TNF-a was enhanced in coculture of lung epithelial cell, MLE-12 and DC, when
compared to culture of each cell alone with Der P. Thus, nanofibrous
scaffold-based 3D coculture system can be very useful to study immune cell
responses to mimic in vivo immune diseases.
Der P. Thus, nanofibrous scaffold-based 3D coculture system can be very useful to study immune cell responses to mimic in vivo immune diseases.
