Changshun Ruan, Ph.D.
Associate professor, Research Center for
Human Tissue and Organs Degeneration, Institute Biomedicine and Biotechnology,
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
cs.ruan@siat.ac.cn
Biography:
Dr. Changshun Ruan is currently an associate
professor in Center for Human Tissue and Organs Degeneration, Institute
Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, CAS.
Dr. Ruan completed his Ph.D. study in Biomedical Engineering at Chongqing
University in 2011. Dr. Ruan ’s research focuses on the development of novel
bioinks for bioprinting of cell-laden constructs for functionalized tissue
regeneration. During the past years, Dr. Ruan has published 40 peer-reviewed
papers and in 22 papers served as first or corresponding author, such as in the journals of Adv. Funct. Mater. (IF=15.621), Adv.
Sci. (IF=15.804) and Biomaterials (IF=10.273).
Topic title：Exploring on Novel
Functional Bioinks Facilitates Bioprinting of 3D Tissue-like Constructs for
Regeneration Medicine
Abstract：The emerging 3D bioprinting
technique that is strongly dependent on the development of bioinks offers a
promising opportunity to customize personalized bioscaffolds for precision and
individualized therapy of tissue defects [1-5]. Although great
progresses have been devoted to imitating the native structure of tissues and
improving the viability of 3D-bioprinted cells in the short term, whether or
not the 3D-bioprinted cells can realize the functional features of tissue still
remains unknown. Indeed, the long-term evaluation of most of the previously
reported cell-laden scaffolds for tissue regeneration is deficient and limited,
due to the lack of ideal inducing bio-inks for bioprinting to favorably support
cell growth and development both in the short and long terms . The
biomaterials which can be used as bio-inks for tissue regeneration should
satisfy the following basic requirements: (1) extrusion properties – suitable
rheology for bioprinting; (2) stability – the printed scaffolds should not
collapse before solidification or crosslinking; (3) biocompatibility and
porosity – high cell viability after printing, with optimal avenues for the
adequate diffusion of nutrients and oxygen to facilitate cell proliferation or
differentiation in the short term; (4) appropriate mechanical properties; and
(5) tissue inducing capability to promote cell differentiation and new tissue
formation in the long term. On basis of these requirements, we currently
developed a series of functional bioinks for facile extrusion bioprinting,
include the modification of alginate/gelatin-based bioinks, high strength
hydrogel-based bioinks and so on.
Acknowledgements:
The authors gratefully acknowledge the support for
this work from the National Key R&D Program of China [grant number
2018YFA0703100], the National Nature Science Foundation of China [Grant No.
31771041], and the Youth Innovation Promotion Association of CAS (Grant No.
2019350).
References:
[1] Zifeng
Lin, Mingming Wu, Huimin He, Qingfei
Liang , Chengshen Hu, Zhiwen Zeng, Delin
Cheng, Goucheng Wang, Dafu Chen,* Haobo Pan,* and Changshun Ruan *. 3D Printing of
Mechanically Stable Calcium-Free Alginate-Based Scaffolds with Tunable Surface
Charge to Enable Cell Adhesion and Facile Biofunctionalization, Advanced Functional Materials, 2019,
1808439.
[2] Fei Gao , Ziyang
Xu , Qingfei Liang, Haofei Li, Liuqi Peng , Mingming Wu , Xiaoli Zhao , Xu Cui, Changshun Ruan* and Wenguang Liu *.
Osteochondral Regeneration with 3D-Printed Biodegradable High-Strength
Supramolecular Polymer Reinforced-Gelatin Hydrogel Scaffolds, Advanced Science. 2019, 1900867. (IF=15.804)
[3] Fei Gao, Changshun Ruan*, Wenguang Liu*.
High-strength Hydrogel-based Bioinks, Materials
Chemistry Frontiers, 2019, 3, 1736.
[4] Fei Gao , Ziyang Xu, Qingfei Liang, Bo Liu ,
Haofei Li, Yuanhao Wu, Yinyu Zhang,
Zifeng Lin, Mingming Wu, Changshun Ruan*, Wenguang Liu *, Direct 3D
Printing of High Strength Biohybrid Gradient Hydrogel Scaffolds for Efficient
Repair of Osteochondral Defect, Advanced
Functional Materials, 2018, 1706644.
[5] Xinyun Zhai , Changshun Ruan*, Yufei Ma , Delin
Cheng, Mingming Wu , Wenguang Liu, Xiaoli Zhao, Haobo Pan*, William Weijia Lu*,
3D-bioprinted Osteoblast-laden Nanocomposite Hydrogel Constructs with Induced
Microenvironments Promote Cell Viability, Differentiation and Osteogenesis both
In Vitro and In Vivo, Advanced Science,
2018, 3:201700550.
